Molecular Assessment of Kappaphycus alvarezii Cultivated in Tarakan based on cox2-3 Spacer

The seaweed Kappaphycus alvarezii is a leading aquaculture commodity possessing high economic value that has been used as raw material for various natural products, food, and pharmaceutical industries. main challenge regarding its production, escpecially in Tarakan the supply of superior seeds. Therefore, this study aims to obtain phylogenetic information cultivation performance selected seeds by identifying genetic sources based on molecular markers. DNA sequencing analysis was carried out using marker cox2-3 spacer collection comprising 16 cultivars from all over country (Indonesia). four haplotypes representing K. be cultivated city were produced clustering further intergenic mitochondrial marker. These include Kupang varieties Natuna, also referred Lampung seedlings, which tissue culture plantlet development at SEAMEO-BIOTROP Bogor propagated Marine Aquaculture Center (BBPBL), Tarakan. genetically distances (pairwise comparisons mean Kimura 2-parameter model) ranging 0.00-0.23 unit. 30 days waters Tanjung Batu (Tj. Batu) Mamburungan, Tarakan, North Kalimantan, days. result showed Natuna* cultivar compared local (Takalar Mamburungan Batu, Kupang). Furthermore, with markers had potential an effective seed selection tool tracing seedlings activities.